1樓:昝昆琦
不同的ph值緩衝液配置方法不一樣,以下可供參考
甘氨酸—鹽酸緩衝液(0.05mol/l)
x ml 0.2mol/l甘氨酸+y ml 0.2mol/l hcl,再加水稀釋至200ml
ph x/ml y/ml ph x/ml y/ml
2.2 50 44.0 3.0 50 11.4
2.4 50 32.4 3.2 50 8.2
2.6 50 24.2 3.4 50 6.4
2.8 50 16.8 3.6 50 5.0
甘氨酸mr = 75.07,0.2mol/l甘氨酸溶液含15.01g/l
2.甘氨酸—氫氧化鈉緩衝液(0.05mol/l)
x ml 0.2mol/l甘氨酸+y ml 0.2mol/l naoh,加水稀釋至200ml
ph x/ml y/ml ph x/ml y/ml
8.6 50 4.0 9.6 50 22.4
8.8 50 6.0 9.8 50 27.2
9.0 50 8.8 10.0 50 32.0
9.2 50 12.0 10.4 50 38.6
9.4 50 16.8 10.6 50 45.5
甘氨酸mr = 75.07,0.2mol/l甘氨酸溶液為15.01g/l
3.鄰苯二甲酸—鹽酸緩衝液(0.05mol/l,20℃)
x ml 0.2mol/l鄰苯二甲酸氫鉀+y ml 0.2mol/l hcl,再加水稀釋到20ml
ph x/ml y/ml ph x/ml y/ml
2.2 5 4.670 3.2 5 1.470
2.4 5 3.960 3.4 5 0.990
2.6 5 3.295 3.6 5 0.597
2.8 5 2.642 3.8 5 0.263
3.0 5 2.032
鄰苯二甲酸氫鉀mr = 204.23,0.2mol/l鄰苯二甲酸氫鉀溶液含40.85g/l
4.檸檬酸—氫氧化鈉—鹽酸緩衝液
ph na+濃度
/mol/l 檸檬酸/g
c6h8o7•h2o 氫氧化鈉/g
naoh(97%) 鹽酸/ml
hcl(濃) 最終體積/l*
2.2 0.20 210 84 160 10
3.1 0.20 210 83 116 10
3.3 0.20 210 83 106 10
4.3 0.20 210 83 45 10
5.3 0.35 245 144 68 10
5.8 0.45 285 186 105 10
6.5 0.38 266 156 126 10
使用時可以每升中加入1g酚,若最後ph值有變化,再用少量50%氫氧化鈉溶液或濃鹽酸調節,置冰箱儲存。
5.磷酸氫二鈉—檸檬酸緩衝液
ph 0.2ol/l na2hpo4/ml 0.1mol/l
檸檬酸/ml ph 0.2mol/l na2hpo4/ml 0.1mol/l
檸檬酸/ml
2.2 0.40 19.60 5.2 10.72 9.28
2.4 1.24 18.76 5.4 11.15 8.85
2.6 2.18 17.82 5.6 11.60 8.40
2.8 3.17 16.83 5.8 12.09 7.91
3.0 4.11 15.89 6.0 12.63 7.37
3.2 4.94 15.06 6.2 13.22 6.78
3.4 5.70 14.30 6.4 13.85 6.15
3.6 6.44 13.56 6.6 14.55 5.45
3.8 7.10 12.90 6.8 15.45 4.55
4.0 7.71 12.29 7.0 16.47 3.53
4.2 8.28 11.72 7.2 17.39 2.61
4.4 8.82 11.18 7.4 18.17 1.83
4.6 9.35 10.65 7.6 18.73 1.27
4.8 9.86 10.14 7.8 19.15 0.85
5.0 10.30 9.70 8.0 19.45 0.55
na2hpo4 mr = 141.98,0.2mol/l溶液為28.40g/l,
na2hpo4•2h2o mr = 178.05,0.2mol/l溶液為35.61g/l
c6h8o7•h2o mr = 210.14,0.1mol/l溶液為21.01g/l
6.檸檬酸—檸檬酸鈉緩衝液(0.1mol/l)
ph 0.1mol/l
檸檬酸/ml 0.1mol/l
檸檬酸鈉/ml ph 0.1mol/l
檸檬酸/ml 0.1mol/l
檸檬酸鈉/ml
3.0 18.6 1.4 5.0 8.2 11.8
3.2 17.2 2.8 5.2 7.3 12.7
3.4 16.0 4.0 5.4 6.4 13.6
3.6 14.9 5.1 5.6 5.5 14.5
3.8 14.0 6.0 5.8 4.7 15.3
4.0 13.1 6.9 6.0 3.8 16.2
4.2 12.3 7.7 6.2 2.8 17.2
4.4 11.4 8.6 6.4 2.0 18.0
4.6 10.3 9.7 6.6 1.4 18.6
4.8 9.2 10.8
檸檬酸c6h8o7•h2o,mr = 210.14,0.1mol/l溶液為21.01g/l
檸檬酸鈉na3c6h5o7•2h2o,mr = 294.12,0.1mol/l溶液為29.41g/l
7.乙酸—乙酸鈉緩衝液(0.2mol/l,18℃)
ph 0.2mol/l
naac/ml 0.2mol/l
hac/ml ph 0.2mol/l
naac/ml 0.2mol/l
hac/ml
3.6 0.75 9.25 4.8 5.90 4.10
3.8 1.20 8.80 5.0 7.00 3.00
4.0 1.80 8.20 5.2 7.90 2.10
4.2 2.65 7.35 5.4 8.60 1.40
4.4 3.70 6.30 5.6 9.10 0.90
4.6 4.90 5.10 5.8 9.40 0.60
naac•3h2o,mr = 136.09,0.2mol/l溶液為27.22g/l
8.鄰苯二甲酸氫鉀—氫氧化鈉緩衝液
50ml 0.1mol/l鄰苯二甲酸氫鉀+x ml 0.1mol/l氫氧化鈉,加水稀釋到100ml
ph x/ml ph x/ml ph x/ml
4.1 1.3 4.8 16.5 5.5 36.6
4.2 3.0 4.9 19.4 5.6 38.8
4.3 4.7 5.0 22.6 5.7 40.6
4.4 6.6 5.1 25.5 5.8 42.3
4.5 8.7 5.2 28.8 5.9 43.7
4.6 11.1 5.3 31.6
4.7 13.6 5.4 34.1
鄰苯二甲酸氫鉀mr = 204.23,0.1mol/l溶液為20.42g/l
9.磷酸鹽緩衝液
(1)磷酸氫二鈉—磷酸二氫鈉緩衝液(0.2mol/l)
ph 0.2mol/l
na2hpo4/ml 0.2mol/l
nah2po4/ml ph 0.2mol/l
na2hpo4/ml 0.2mol/l
nah2po4/ml
5.8 8.0 92.0 7.0 61.0 39.0
5.9 10.0 90.0 7.1 67.0 33.0
6.0 12.3 87.7 7.2 72.0 28.0
6.1 15.0 85.0 7.3 77.0 23.0
6.2 18.5 81.5 7.4 81.0 19.0
6.3 22.5 77.5 7.5 84.0 16.0
6.4 26.5 73.5 7.6 87.0 13.0
6.5 31.5 68.5 7.7 89.5 10.5
6.6 37.5 62.5 7.8 91.5 8.5
6.7 43.5 56.5 7.9 93.0 7.0
6.8 49.0 51.0 8.0 94.7 5.3
6.9 55.0 45.0
na2hpo4•2h2o,mr = 178.05,0.2mol/l溶液為35.61g/l
na2hpo4•12h2o,mr = 358.22,0.2mol/l溶液為71.64g/l
nah2po4•h2o,mr = 138.01,0.2mol/l溶液為27.6g/l
nah2po4•2h2o,mr = 156.03,0.2mol/l溶液為31.21g/l
(2)磷酸氫二鈉—磷酸二氫鉀緩衝液(1/15mol/l)
ph 1/15mol/l
na2hpo4/ml 1/15mol/l
kh2po4/ml ph 1/15mol/l
na2hpo4/ml 1/15mol/l
kh2po4/ml
4.92 0.10 9.90 7.17 7.00 3.00
5.29 0.50 9.50 7.38 8.00 2.00
5.91 1.00 9.00 7.73 9.00 1.00
6.24 2.00 8.00 8.04 9.50 0.50
6.47 3.00 7.00 8.34 9.75 0.25
6.64 4.00 6.00 8.67 9.90 0.10
6.81 5.00 5.00 8.18 10.00 0
6.98 6.00 4.00
na2hpo4•2h2o,mr = 178.05,1/15mol/l溶液為11.876g/l
kh2po4,mr = 136.09,1/15mol/l溶液為9.078g/l
10.磷酸氫二鈉—氫氧化鈉緩衝液
50ml 0.05mol/l磷酸氫二鈉+x ml 0.1mol/l naoh,加水稀釋至100ml
ph x/ml ph x/ml ph x/ml
10.9 3.3 11.3 7.6 11.7 16.2
11.0 4.1 11.4 9.1 11.8 19.4
11.1 5.1 11.5 11.1 11.9 23.0
11.2 6.3 11.6 13.5 12.0 26.9
na2hpo4•2h2o,mr = 178.05,0.05mol/l溶液為8.90g/l
na2hpo4•12h2o,mr = 358.22,0.05mol/l溶液為17.91g/l
11.磷酸二氫鉀—氫氧化鈉緩衝液(0.05mol/l,20℃)
x ml 0.2mol/l kh2po4+y ml 0.2mol/l naoh,加水稀釋至20ml
ph x/ml y/ml ph x/ml y/ml
5.8 5 0.372 7.0 5 2.963
6.0 5 0.570 7.2 5 3.500
6.2 5 0.860 7.4 5 3.950
6.4 5 1.260 7.6 5 4.280
6.6 5 1.780 7.8 5 4.520
6.8 5 2.365 8.0 5 4.680
12.硼砂—鹽酸緩衝液(0.05mol/l硼酸根)
50ml 0.025mol/l硼砂+x ml 0.1mol/l 鹽酸,加水稀釋至100ml
ph x/ml ph x/ml ph x/ml
8.00 20.5 8.4 16.6 8.8 9.4
8.10 19.7 8.5 15.2 8.9 7.1
8.20 18.8 8.6 13.5 9.0 4.6
8.30 17.7 8.7 11.6 9.1 2.0
硼砂na2b4o7•10h2o,mr = 381.43,0.025mol/l溶液為9.53g/l
13.巴比妥鈉—鹽酸緩衝液(18℃)
ph 0.04mol/l
巴比妥鈉溶液/ml 0.2mol/l
鹽酸/ml ph 0.04mol/l
巴比妥鈉溶液/ml 0.2mol/l
鹽酸/ml
6.8 100 18.4 8.4 100 5.21
7.0 100 17.8 8.6 100 3.82
7.2 100 16.7 8.8 100 2.52
7.4 100 15.3 9.0 100 1.65
7.6 100 13.4 9.2 100 1.13
7.8 100 11.47 9.4 100 0.70
8.0 100 9.39 9.6 100 0.35
8.2 100 7.21
巴比妥鈉鹽mr = 206.18,0.04mol/l溶液為8.25g/l
14.tris—鹽酸緩衝液(0.05mol/l,25℃)
50ml 0.1mol/l三羥甲基氨基甲烷(tris)溶液與x ml 0.1mol/l 鹽酸混勻後,加水稀釋至100ml
ph x/ml ph x/ml
7.10 45.7 8.10 26.2
7.20 44.7 8.20 22.9
7.30 43.4 8.30 19.9
7.40 42.0 8.40 17.2
7.50 40.3 8.50 14.7
7.60 38.5 8.60 12.4
7.70 36.6 8.70 10.3
7.80 34.5 8.80 8.5
7.90 32.0 8.90 7.0
8.00 29.2 9.00 5.7
注:三羥甲基氨基甲烷(tris)
mr = 121.14,0.1mol/l溶液為12.114g/l。tris溶液可從空氣中吸收二氧化碳,儲存時注意密封。
15.硼酸—硼砂緩衝液(0.2mol/l硼酸根)
ph 0.05mol/l
硼砂/ml 0.2mol/l
硼酸/ml ph 0.05mol/l
硼砂/ml 0.2mol/l
硼酸/ml
7.4 1.0 9.0 8.2 3.5 6.5
7.6 1.5 8.5 8.4 4.5 5.5
7.8 2.0 8.0 8.7 6.0 4.0
8.0 3.0 7.0 9.0 8.0 2.0
硼砂na2b4o7•10h2o,mr = 381.43,0.05mol/l溶液(=0.2 mol/l硼酸根)為19.07g/l,硼砂易失去結晶水,必須密閉儲存
硼酸h3bo3,mr = 61.84,0.2mol/l溶液為12.37g/l
16.硼砂—氫氧化鈉緩衝液(0.05mol/l硼酸根)
x ml 0.05mol/l硼砂+y ml 0.2mol/l naoh,加水稀釋至200ml
ph x/ml y/ml ph x/ml y/ml
9.3 50 6.0 9.8 50 34.0
9.4 50 11.0 10.0 50 43.0
9.6 50 23.0 10.1 50 46.0
硼砂na2b4o7•10h2o,mr = 381.43,0.05mol/l溶液為19.07g/l
17.碳酸鈉—碳酸氫鈉緩衝液(0.1mol/l)
ca2+、mg2+存在時不得使用
ph 0.1mol/l碳酸鈉/ml 0.1mol/l碳酸氫鈉/ml
20℃ 37℃
9.16 8.77 1 9
9.40 9.12 2 8
9.51 9.40 3 7
9.78 9.50 4 6
9.90 9.72 5 5
10.14 9.90 6 4
10.28 10.08 7 3
10.53 10.28 8 2
10.83 10.57 9 1
無水碳酸鈉mr = 105.99,0.1mol/l溶液為10.60g/l
碳酸氫鈉mr = 84.01,0.1mol/l溶液為8.40g/l
18.碳酸氫鈉—氫氧化鈉緩衝液(0.025mol/l碳酸氫鈉)
50ml 0.05mol/l碳酸氫鈉+x ml 0.1mol/l naoh,加水稀釋至100ml
ph x/ml ph x/ml ph x/ml
9.6 5.0 10.1 12.2 10.6 19.1
9.7 6.2 10.2 13.8 10.7 20.2
9.8 7.6 10.3 15.2 10.8 21.2
9.9 9.1 10.4 16.5 10.9 22.0
10.0 10.7 10.5 17.8 11.0 22.7
碳酸氫鈉mr = 84.01;0.05mol/l溶液為4.20g/l
19.氯化鉀—氫氧化鈉緩衝液
25ml 0.2mol/l kcl+x ml 0.2mol/l naoh,加水稀釋至100ml
ph x/ml ph x/ml ph x/ml
12.0 6.0 12.4 16.2 12.8 41.2
12.1 8.0 12.5 20.4 12.9 53.0
12.2 10.2 12.6 25.6 13.0 66.0
12.3 12.8 12.7 32.2
kcl mr = 74.55;0.2mol/l溶液為14.91g/l
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